Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding

Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target...

متن کامل

Continuous Microfluidic Immunosensing with Antibody Conjugated Paramagnetic Beads

A microdevice assay is being developed to continuously measure specific antigen concentrations within a sample stream. This device will allow real-time measurement of systemic protein concentrations within blood plasma, at high sample rates not possible with existing bench-top assays. Magnetic actuation of antibody coated paramagnetic microbeads between sample and wash streams leads to highly c...

متن کامل

Microfluidic capturing-dynamics of paramagnetic bead suspensions.

We study theoretically the capturing of paramagnetic beads by a magnetic field gradient in a microfluidic channel treating the beads as a continuum. Bead motion is affected by both fluidic and magnetic forces. The transfer of momentum from beads to the fluid creates an effective bead-bead interaction that greatly aids capturing. We demonstrate that for a given inlet flow speed a critical densit...

متن کامل

Ultrasensitive proteome analysis using paramagnetic bead technology

In order to obtain a systems-level understanding of a complex biological system, detailed proteome information is essential. Despite great progress in proteomics technologies, thorough interrogation of the proteome from quantity-limited biological samples is hampered by inefficiencies during processing. To address these challenges, here we introduce a novel protocol using paramagnetic beads, te...

متن کامل

Kinetics of cell death triggered photothermally using folate-conjugated gold nanoparticles and various laser irradiation conditions in cancer cells

Introduction: In this study, we explore in detail cell-specific targeting efficacy of nano-photo-thermal therapy (NPTT) method and the resulting responses that are induced by variable laser intensities and exposure times in cancer cells to induce selective apoptosis. We delineate the synthesis of a high-yielding synthetic F-AuNPs by tailoring the surface of gold nanoparticles ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: International Journal of Molecular Sciences

سال: 2014

ISSN: 1422-0067

DOI: 10.3390/ijms15058821